abstract：:Neurite outgrowth is dependent on the presence in neurites of assembled microtubules which consist of polymerized tubulin to which microtubule-associated proteins (MAPs) are bound. This study adds to previous evidence of the similarity between the high molecular weight MAPs, MAP1X, MAP1B and MAP5, and explains the dif...

journal_title：Developmental neuroscience

authors： Harrison L,Cheetham ME,Calvert RA

更新日期：1993-01-01 00:00:00

abstract：:In the development of an integrated approach to study metabolic compartmentation and regulation in brain, we have emphasized the importance, versatility, and need to exploit the recent methodological advances in (1) NMR spectroscopy, (2) primary cultures of neurons and glial cells, and (3) subcellular fractionation (e...

journal_title：Developmental neuroscience

authors： Lai JC,Behar KL

更新日期：1993-01-01 00:00:00

abstract：:The metabolism of [1-13C]D-glucose by astrocytes, neurons and mixed astroglial/neuronal cultures derived from the striatum of fetal rats was studied using NMR. Metabolic activity was studied in resting and depolarized cells (55 mM K+), with dibutyryl cyclic-AMP added to the medium to promote cell differentiation, and ...

journal_title：Developmental neuroscience

authors： Leo GC,Driscoll BF,Shank RP,Kaufman E

更新日期：1993-01-01 00:00:00

abstract：:Metabolism of glutamate and glutamine in cultured mouse cerebral cortical astrocytes has been investigated using either radioactively labelled (14C) amino acids or 13C-labelled amino acids combined with NMR spectroscopy of cell extracts and lyophilyzed incubation media. Using [U-13C]glutamate it has been shown that in...

journal_title：Developmental neuroscience

authors： Schousboe A,Westergaard N,Sonnewald U,Petersen SB,Huang R,Peng L,Hertz L

更新日期：1993-01-01 00:00:00

abstract：:L-Ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine biosynthesis and thus a unique marker of the proliferation and maturation processes, has been localized by means of immunohistochemistry in the human central nervous system. The enzyme protein has not only been found in the developing nervous syste...

journal_title：Developmental neuroscience

authors： Müller M,Pajunen AE,Järvinen M,Poeggel G,Bernstein HG

更新日期：1993-01-01 00:00:00

abstract：:Developmental changes in gangliosides in the course of neurite outgrowth were examined in dissociated fetal rat cerebral neurons in culture. About a 2-fold increase in ganglioside levels was seen with the progression of neurite formation for up to 24 h in predominantly neuronal cultures. Ganglioside patterns appeared ...

journal_title：Developmental neuroscience

authors： Ogiso M,Ohta M,Harada Y,Hirano S

更新日期：1992-01-01 00:00:00

abstract：:When cells from the cerebellum of late-gestation rats were grown at low density (50-100 cells/mm2) in a three-dimensional culture system, they rapidly expressed morphological and electrophysiological properties of neurons. Growth and differentiation of the population of solitary neurons as a whole was statistically as...

journal_title：Developmental neuroscience

authors： Coates PW,Fermini B,Strahlendorf JC,Strahlendorf HK

更新日期：1992-01-01 00:00:00

abstract：:Primary cultures of cerebellum from 5-day-old rats indicated that proliferating neuroblasts synthesize and release nerve growth factor (NGF). Since NGF promotes DNA synthesis in these cells, our findings demonstrate that the early developing cerebellum is a suitable physiological model for studying the autocrine mitog...

journal_title：Developmental neuroscience

authors： Charrasse S,Jehan F,Confort C,Brachet P,Clos J

更新日期：1992-01-01 00:00:00

abstract：:Quaking is a neurological mutation leading to pleiotropic phenotypic expression, the most prominent being disturbed myelin formation in the central nervous system (CNS) with minor abnormalities in the peripheral nervous system. Previous immunochemical measurements of glial fibrillary acidic protein (GFAP) revealed a m...

journal_title：Developmental neuroscience

authors： Chen H,Sun P,Parmantier E,Cabon F,Dupouey P,Zalc B,Jacque C

更新日期：1992-01-01 00:00:00

abstract：:We have previously reported that a developmental axon-sparing striatal injury induced by hypoxia-ischemia results in a diminished adult number of substantia nigra (SN) tyrosine hydroxylase (TH)-positive neurons, in the absence of direct nigral injury. To examine the specific role of striatal injury, we made selective ...

journal_title：Developmental neuroscience

authors： Macaya A,Burke RE

更新日期：1992-01-01 00:00:00

abstract：:Fragments of corpus callosum from P13 normal and jimpy (jp) mutant mice (containing only postmigrating precursors and differentiated oligodendrocytes (ODCs, some of them myelinating soon) have been transplanted into the thalamus of newborn shiverer (shi) mutant mice. The behaviour of transplanted ODCs has been assayed...

journal_title：Developmental neuroscience

authors： Lachapelle F,Lapie P,Gumpel M

更新日期：1992-01-01 00:00:00

abstract：:Previous studies have demonstrated that exposure to ethanol during development delays the rate at which axons in certain central nervous system tracts acquire myelin. This delay appears to be related to an alteration in oligodendrocyte function and not to an aberrancy in axon size or number. The present study was desi...

journal_title：Developmental neuroscience

authors： McNeill DL,Shew RL,Papka RE

更新日期：1991-01-01 00:00:00

abstract：:The myelin-associated glycoprotein (MAG) in primary cultures of oligodendrocytes is subject to phosphorylation in the absence of neurons. The positive response of this phosphorylation to vanadate suggests that one of the modified sites in MAG is phosphotyrosine. We found that phosphorylation was enhanced by brief trea...

journal_title：Developmental neuroscience

authors： Bambrick LL,Braun PE

更新日期：1991-01-01 00:00:00

abstract：:All known actions of cAMP in the brain require cAMP-dependent protein kinase (cAMPdPK), which consists of regulatory (R) and catalytic (C) subunits (R2C2). Using homologous rat cDNAs for all known cAMPdPK subunit isoforms found in the brain (RI alpha, RI beta, RII alpha, RII beta, C alpha, C beta) we observe that, in ...

journal_title：Developmental neuroscience

authors： Massa JS,Walker PS,Moser DR,Fellows RE,Maurer RA

更新日期：1991-01-01 00:00:00

abstract：:Adult GM2 gangliosidosis is a rare autosomal recessive disease with widely varying neurological and psychiatric manifestations. It is caused by marked deficiency, but not total absence, of beta-hexosaminidase (Hex) A, due to a single base change in the alpha-subunit gene of Hex, resulting in a substitution of Ser for ...

journal_title：Developmental neuroscience

authors： Navon R

更新日期：1991-01-01 00:00:00

abstract：:Parallel developmental studies of central nervous system myelin proteins and morphology (postnatal days 15-118; P15-118) confirm qualitative similarities but substantial quantitative differences between homozygous mld mice with Billings-Gagliardi and Wolf's 'USA' versus Matthieu's 'Swiss' genetic backgrounds. The USA ...

journal_title：Developmental neuroscience

authors： Billings-Gagliardi S,Tosic M,Matthieu JM,Kirschner DA,Wolf MK

更新日期：1991-01-01 00:00:00

abstract：:Adrenoleukodystrophy (ALD) is an X-linked recessive disorder that affects mainly the nervous system white matter and the adrenal cortex. It is associated with an abnormal accumulation of saturated very long chain fatty acids and can be diagnosed by demonstrating an excess of these substances in plasma or red cells. Ou...

journal_title：Developmental neuroscience

authors： Moser HW,Moser AB,Naidu S,Bergin A

更新日期：1991-01-01 00:00:00

abstract：:Adult neuronal ceroid lipofuscinosis (NCL), also called Kufs' disease, is clinically distinct from the other NCLs. It is a rare condition which is difficult to diagnose. More than 50% of the reported cases of Kufs' disease are not adult NCL and correspond very likely to a heterogeneous spectrum of lipidoses. Various c...

journal_title：Developmental neuroscience

authors： Martin JJ

更新日期：1991-01-01 00:00:00

abstract：:Insulin binding was measured on crude mitochondrial or plasma membranes prepared from different rat brain regions during postnatal development. In the cerebral cortex and brainstem, insulin binding decreased 60-70% between birth and the adult period. In the cerebellum, insulin binding doubled in the first 10 postnatal...

journal_title：Developmental neuroscience

authors： Marks JL,Eastman CJ

更新日期：1990-01-01 00:00:00

abstract：:We have shown that the developmentally regulated appearance of the two myelin-associated glycoprotein (MAG) polypeptides in normal mouse brain myelin does not reflect the developmental pattern of differential splicing of primary gene transcripts as determined earlier by RNase protection assays. Contrary to expectation...

journal_title：Developmental neuroscience

authors： Braun PE,Horvàth E,Edwards AM

更新日期：1990-01-01 00:00:00

abstract：:The distal segments of the crush-injured and permanently transected sciatic nerve provide models to study Schwann cell activity in the presence and absence of Schwann cell-axonal contact, respectively. We examined the quantity and quality of transcript coding for the myelin-associated glycoprotein (MAG) over a 3-week ...

journal_title：Developmental neuroscience

authors： Gupta SK,Poduslo JF,Dunn R,Roder J,Mezei C

更新日期：1990-01-01 00:00:00

abstract：:The activity of glycerophosphorylcholine phosphodiesterases was determined in the mesencephalon, diencephalon, cerebral hemispheres, cerebellum and olfactory bulb during postnatal development from P5 to P70 of rat brain. These activities are low and gradually increase to near adult levels by the end of the first postn...

journal_title：Developmental neuroscience

authors： Kanfer JN,McCartney DG

更新日期：1989-01-01 00:00:00

abstract：:We have used whole-cell and single-channel recording techniques to investigate the electrophysiological properties of cultured ovine oligodendrocytes (OLGs). Our studies have led to the following conclusions. (1) Cultured mature OLGs express a variety of voltage-dependent K+ conductances including an outward current t...

journal_title：Developmental neuroscience

authors： Soliven B,Szuchet S,Arnason BG,Nelson DJ

更新日期：1989-01-01 00:00:00

abstract：:Rabbit central (CNS) and peripheral nervous system (PNS) myelin, as well as nonmyelinating pig oligodendrocytes in culture, were extracted at 0-4 degrees C with the nonionic detergent Triton X-114. The solubilized proteins were partitioned into the detergent-rich and detergent-depleted (aqueous) phases that form upon ...

journal_title：Developmental neuroscience

authors： Bürgisser P,Matthieu JM

更新日期：1989-01-01 00:00:00

abstract：:Choline acetyltransferase (ChAT), the biosynthetic enzyme for acetylcholine metabolism, is expressed in a human cholinergic neuroblastoma cell line; MC-IXC. We demonstrate that ChAT activity is regulated in this cell line by cell density. It is believed that the mechanism of stimulation of enzyme activity involves cel...

journal_title：Developmental neuroscience

authors： Casper D,Davies P

更新日期：1988-01-01 00:00:00

abstract：:Cultures of rat oligodendrocytes were used to test the possible role of the cerebellar soluble lectin (CSL) in myelin formation. Immunocytochemistry at the ultrastructural level showed that the lectin is present in the cytoplasm of the perikaryon of cultured oligodendrocytes and also on the plasma membrane of the cell...

journal_title：Developmental neuroscience

authors： Kuchler S,Fressinaud C,Sarlieve LL,Vincendon G,Zanetta JP

更新日期：1988-01-01 00:00:00

abstract：:A glia-derived neurite-promoting factor has been purified from medium conditioned by C6 rat glioma cells. It induces neurite outgrowth in cultured mouse neuroblastoma cells and inhibits granule cell migration in explants of mouse cerebellum. This factor is a potent serine protease inhibitor which has recently been sho...

journal_title：Developmental neuroscience

authors： Zurn AD,Nick H,Monard D

更新日期：1988-01-01 00:00:00

abstract：:The lipids of white matter and peripheral nerve from mutant mice with known myelin deficiencies were analyzed by one- and two-dimensional high-performance thin-layer chromatography and quantitated by densitometry. In optic nerve, the mutants jp/Y, jpmsd/Y, qk/qk, shi/shi and shimld/shimld, which have severe central ne...

journal_title：Developmental neuroscience

authors： Ganser AL,Kerner AL,Brown BJ,Davisson MT,Kirschner DA

更新日期：1988-01-01 00:00:00

abstract：:The cytoskeleton of a newly isolated mouse embryo brain-derived cell line and of dissected mesencephalon-rhombencephalon samples of the 10- to 11-day-old mouse embryo, constituted of a ventricular zone only, has been examined by immunocytochemistry, electrophoretic separation and Western blotting techniques. In accord...

journal_title：Developmental neuroscience

authors： Zákány J,Murach KF,Fundele R,Illmensee K

更新日期：1987-01-01 00:00:00

abstract：:The cerebromicrovasculature of immature mice ranging in age from 1-24 days after birth was studied by electron microscopy. A micromanipulator apparatus enabled us to facilitate direction of a needle cannula and injection into leg veins or hearts with horseradish peroxidase (HRP) or ferritin (anionic and cationic) trac...

journal_title：Developmental neuroscience

authors： Lossinsky AS,Vorbrodt AW,Wisniewski HM

更新日期：1986-01-01 00:00:00

abstract：:Astrocytes in primary cultures constitute an exceedingly useful preparation for studies of astroglial development and function. These cells, however, demonstrate a pronounced plasticity in their reactions to culturing conditions. Thus, species and spatiotemporal region of CNS chosen for source of cells, dissociation p...

journal_title：Developmental neuroscience

authors： Juurlink BH,Hertz L

更新日期：1985-01-01 00:00:00

abstract：:Extracts prepared from embryonic, neonatal and adult rat brain were examined for the presence of oligodendroglial mitogens. Brain-derived mitogenic activities were found in all developmental stages with specific activity increasing during neonatal development. Extracts from postnatal day 7 contained the highest specif...

journal_title：Developmental neuroscience

authors： Saneto RP,de Vellis J

更新日期：1985-01-01 00:00:00

abstract：:Disorders primarily affecting the nervous system comprise approximately one third of all established Mendelian genetic diseases in man. Recombinant DNA technology provides new approaches to the diagnosis and elucidation of the molecular pathology of these disorders. For a small but increasing number of disorders the D...

journal_title：Developmental neuroscience

authors： Hayden MR,Nichols JL

更新日期：1983-01-01 00:00:00

abstract：:The myelin-associated glycoprotein (MAG) is a 100 K-dalton, integral membrane glycoprotein containing 30% carbohydrate that is in central and peripheral myelin sheaths. Recent research on its function during normal myelinogenesis and possible roles in the pathology of demyelinating diseases is reviewed. Emphasis is pl...

journal_title：Developmental neuroscience

authors： Quarles RH

更新日期：1983-01-01 00:00:00

abstract：:Several studies have shown that exogenous gangliosides can stimulate neurite outgrowth (in vitro), accelerate peripheral nerve regeneration (in vivo) and facilitate CNS recovery after lesioning. Experiments were designed to assess the effects of ganglioside administration on neonatal development. Rat neonates received...

journal_title：Developmental neuroscience

authors： Karpiak SE,Vilim F,Mahadik SP

更新日期：1983-01-01 00:00:00

abstract：:In this study, the proliferative activity of cultured chick neuroblasts exposed to high or low concentrations of fetal calf serum (FCS) was investigated. Different experimental evidences showed that the first 2-3 days of culture correspond to a period of neuronal proliferation in our culture conditions, whereas contam...

journal_title：Developmental neuroscience

authors： Barakat I,Labourdette G,Sensenbrenner M

更新日期：1983-01-01 00:00:00

abstract：:The kinetics and sodium-dependence of gamma-aminobutyric acid (GABA), hypotaurine and taurine uptake were analyzed in cerebral slices prepared from mice of different postnatal ages. The maximal velocities of transport (V) and the Km constants for the high-affinity uptake all decreased with age, whereas the development...

journal_title：Developmental neuroscience

authors： Oja SS,Kontro P

更新日期：1983-01-01 00:00:00

abstract：:In the presence of nerve growth factor (NGF) PC12 pheochromocytoma cells develop properties of sympathetic neurons and extend long microtubule-containing neurites. PC12 cell microtubule protein was isolated using an assembly and disassembly procedure, either directly from an unlabeled cell extract or by copolymerizati...

journal_title：Developmental neuroscience

authors： Richter-Landsberg C,Landreth GE,Shooter EM

更新日期：1983-01-01 00:00:00

abstract：:In order to investigate the possible influence of norepinephrine (NE) on neuronal development, the effects of neonatal NE depletion on postnatally developing cell populations were characterized in (1) the normal mouse cerebellum, and (2) a cerebellar model system with delayed germinal cell development produced by neon...

journal_title：Developmental neuroscience

authors： Lovell KL

更新日期：1982-01-01 00:00:00

abstract：:Neuron-specific enolase (NSE) is a unique form of the glycolytic enzyme, enolase, which, in the brain, is strictly localized in differentiated neurons and neuroendocrine cells. In this report evidence is presented that NSE is first seen in the cell bodies of developing chick auditory and vestibular neurons shortly aft...

journal_title：Developmental neuroscience

authors： Whitehead MC,Marangos PJ,Connolly SM,Morest DK

更新日期：1982-01-01 00:00:00